| Abstract: | Regenerated silk fibroin (RSF)/graphene oxide (GO) nanocomposite has been substantially investigated due to its significant multifunctional potential. Here, in combination of micromorphology, crystalline conformation, dynamic mechanical property characterization, and Fourier self‐deconvolution (FSD) quantitative analysis, we investigated the RSF molecular chains conformation transition induced by GO nanosheet incorporation, and its influence on the structural and mechanical properties of solution casted RSF/GO composite films. The GO nanosheet promoted the silk fibroin molecular chains conformation transition from random coil to β‐sheet structure, and a correlation between β‐sheet structure fraction and GO concentration was revealed. The β‐sheet structure fraction increases further improved the dynamic mechanical property of composite films. Moreover, based on nucleation‐dependent aggregation of silk fibroin molecular chains, a mechanism considering the competition effect between GO concentration and its total surface area was proposed to explain the observed concentration‐dependent conformation transition phenomenon. The study improves our understanding on silk fibroin conformation transition process in RSF/GO composite and would provide a valuable reference for the rational design of bioinspired multifunctional materials with enhanced mechanical properties. © 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2019 , 57, 1506–1515 |
