| Abstract: | In this study, MnFe2O4 nanoclusters were prepared as bioprobes to establish a lateral‐flow immunochromatographic assay (LFIA) for the rapid and quantitative detection of D‐dimer for the first time. The magnetic properties of the magnetic labels play a key role in the quantitative detection of biomolecules. The 47.3‐nm MnFe2O4 magnetic nanoclusters (MNCs) with good dispersion and high saturation magnetization (76 emu/g) were fabricated via thermal decomposition of Fe(acac)3 with Mn(acac)2. The prepared MnFe2O4 MNCs were well dispersed in water because the surfaces were fully covered with 3,4‐dihydroxyhydrocinnamic acid (DHCA) molecules by ligand exchange. Anti‐D‐dimer antibodies were coupled on the surface of MnFe2O4 MNCs, and the target protein, D‐dimer, was detected, in the range 0.05–6 μg/mL. This assay provides a promising platform for D‐dimer detection for point‐of‐care diagnosis. |
