Fluorescent Dye Incorporated Magnetic/Silica and Fluorescent Silica Nanoparticles Based Myoglobin Detection from Whole Blood Samples |
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| Authors: | Kaliyaperumal Viswanathan Y. C. Lee Zhuang Ming Chen |
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| Affiliation: | 1. Department of Animal Biotechnology, Tamilnadu Veterinary and Animal Science University, Madhavaram Milk Colony, Chennai 600 051, India;2. Department of Applied Chemistry, National Chinan University, No.1, University Rd, Puli, Nantou County 54561, Taiwan (R.O.C.) |
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| Abstract: | The anti myoglobin conjugated iron oxide/ru(bpy)32+/silica, anti myoglobin conjugated rhodamine 6G/silica particles were prepared, and used for myoglobin detections. The anti myoglobin conjugated iron oxide/ru(bpy)32+/silica particles were mixed with myoglobin in the micro centrifuge tube. After 10 min, the magnetic separators were introduced to catch the myoglobin‐captured anti myoglobin/iron oxide/ru(bpy)32+/silica particles from the homogenous solutions. For sandwich assays, the anti myoglobin conjugated rhodamine 6G/silica particles were incubated. The concentration of myoglobin was quantified based on anti myoglobin conjugated rhodamine 6G/silica particles. The calibration curve of the myoglobin showed the linear range to be between 1 × 10?12 and 1 × 10?10 (M) (R2 = 0.9944). The minimum detectable concentration was 17.6 pg/mL. The fluorescence method offered the best way to determine myoglobin with a total analysis time of less than 30 min. |
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| Keywords: | Fluorescent nanoparticle Myoglobin Sand wich assay |
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