Rapid Quantification of Efavirenz in Human Plasma by Electrospray Ionization Tandem Mass Spectrometry |
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| Authors: | Suresh Kumar Kailasa Hui‐Fen Wu |
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| Institution: | 1. Department of Chemistry, National Sun Yat‐Sen University, Kaohsiung, 80424, Taiwan;2. Center for Nanoscience and Nanotechnology, National Sun Yat‐Sen University, Kaohsiung, 80424, Taiwan;3. Department of Chemistry, S. V. National Institute of Technology, Surat — 395007, India;4. Doctoral Degree Program in Marine Biotechnology, National Sun Yat‐Sen University, Kaohsiung 80424, Taiwan;5. School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 806, Taiwan;6. Institute of Medical Science and Technology, National Sun Yat‐Sen University, Kaohsiung, 80424, Taiwan |
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| Abstract: | We report a rapid and sensitive method for detecting efavirenz in human plasma by electrospray ionization tandem mass spectrometry (ESI‐MS/MS). Detection of efavirenz in human plasma samples was confirmed by acquiring MS/MS of protonated efavirenz at m/z 316.5 to yield the characteristic fragment ions at m/z 298, 274, 272, 251, 246, 237, 232, 203 and 168, respectively. This approach provided good linearity for the quantification of efavirenz (R2 = ～0.9989), with good precision (RSD >10%) and limit of detection (0.20 nM). This method can be widely applied for precise quantitative analysis of a variety of drugs in clinical samples. |
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| Keywords: | Efavirenz Human plasma ESI‐MS |
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