| 纳米金三明治结构调制细胞色素c电子传递特性的分子机理研究 |
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| 引用本文： | 蔺首睿,王立旭,姜秀娥,郭黎平. 纳米金三明治结构调制细胞色素c电子传递特性的分子机理研究[J]. 电化学, 2012, 0(3): 264-269 |
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| 作者姓名： | 蔺首睿 王立旭 姜秀娥 郭黎平 |
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| 作者单位： | 中国科学院长春应用化学研究所电分析化学国家重点实验室;东北师范大学化学学院 |
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| 基金项目： | 吉林省青年基金项目(No.201101081);中国科学院院长基金资助 |
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| 摘 要： | 以细胞色素c(Cyt c)为模型蛋白,采用表面增强红外吸收光谱监测了三明治结构所吸附的纳米金对氧化还原诱导的Cyt c表面增强红外差谱的改变.研究表明,在单层Cyt c分子表面组装纳米金,使得血红素的红外差谱特征峰明显增强,这归因于纳米金和血红素之间的电子传递.纳米金与Cyt c氧化还原活性中心血红素的相互作用加速了蛋白质的电子传递.这为实现并优化表面吸附蛋白质的直接电化学提供了一种新技术.
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| 关 键 词： | 细胞色素c 纳米金 纳米三明治结构 表面增强红外吸收光谱 电子传递 |
Studies on the Molecular Mechanism of Electron Transfer of Cytochrome c Modulated by Gold Nanoparticles in Nano-Sandwich Architecture |
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| LIN Shou-rui,WANG Li-xu,JIANG Xiu-e,GUO Li-ping. Studies on the Molecular Mechanism of Electron Transfer of Cytochrome c Modulated by Gold Nanoparticles in Nano-Sandwich Architecture[J]. Electrochemistry, 2012, 0(3): 264-269 |
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| Authors: | LIN Shou-rui WANG Li-xu JIANG Xiu-e GUO Li-ping |
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| Affiliation: | 1.State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun 130022,China; 2.Department of Chemistry,Northeast Normal University,Changchun 130024,China) |
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| Abstract: | The effects of adsorbed gold nanoparticles on the surface-enhanced infrared absorption(SEIRA) difference spectra of redox-induced cytochrome(Cyt) c in AuNPs/Cyt c/Au sandwich architecture were monitored by SEIRA spectroscopy.The results indicated that the intensity of SEIRA difference spectrum for the vibration of the heme was significantly increased due to the adsorption of gold nanoparticles on the Cyt c.This was induced by the electron transfer between the heme and the gold nanoparticles,which also promoted electron transfer of adsorbed protein.This study suggested a new technique for optimizing the electrochemical property of adsorbed protein. |
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| Keywords: | Cytochrome c gold nanoparticles nano-sandwich architechture surface-enhanced infrared absorption spectroscopy electron transfer |
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