单细胞分析的研究

单细胞分析是分析化学、生物学和医学之间渗透发展形成的跨学科前沿领域。近年来,毛细管电泳及微流控芯片用于单细胞分析已取得显著进展,特别表现在微流控芯片用于细胞的培养、分选、操纵、定位、分离及检测细胞的组分,实时监测细胞释放,及高通量阵列检测等方面。芯片的单元操作可根据需要灵活组合,显示出其独特的优点。本文重点介绍作者研究组的工作,并对近三年来国内外在毛细管电泳及芯片毛细管电泳用于单细胞分析的新进展进行评论。最后从毛细管电泳与微流控芯片、微流控芯片与细胞界面以及量子点用于探测活细胞等方面,展望了单细胞分析的发展前景。

Studies on Single-Cell Analysis

The single-cell analysis is a frontier field of sciences that has been developed from the interdisciplinary collaborations of analytical chemistry,biology and medicine.At the present time,the great achievements in the application of capillary electrophoresis and microfluidic chip to single-cell analysis,particularly,in the application of microfluidic chip to culture,manipulation,location, separation and detection of components of single cells,real-time detection of secretion from single-cells and high-throughput array detection have been made.The unit procedures can be made up in any device in accordance with the requirements.In this paper,the work of the author's group is presented mainly.In addition,the developments of the capillary electrophoresis and chip electrophoresis applied to single-cell analysis in recent years are reviewed.The prospects for single-cell analysis including capillary electrophoresis and microfluidic chip,measurement and control at the cell-chip interface,and quantum dots for probing live cells are also proposed.