基于废弃固定化酶再利用的高容量强阳离子交换树脂及其对溶菌酶的吸附性能

采用表面引发-原子转移自由基聚合( SI-ATRP)技术,以废弃的固定化酶为基质、4-乙烯基苯磺酸钠为单体,30℃聚合3 h,制备了一种新型强阳离子交换树脂。采用傅里叶变换红外光谱( FT-IR)对合成的强阳离子交换树脂进行表征。以溶菌酶为模型蛋白,考察了溶菌酶初始浓度、离子强度、有机溶剂浓度、吸附时间及温度对吸附容量的影响。结果表明,强阳离子交换树脂对溶菌酶的吸附是一个放热的过程。在室温下对溶菌酶的最大吸附量可达240 mg/g,在30 min内快速达到吸附平衡,比文献报道的阳离子交换树脂具有更好的吸附性能。 Langmuir吸附模型与准二级动力学方程可以较好地对吸附过程进行拟合。

High-Capacity Strong Cation Exchange Resin Based on Reutilization of An Inactivated Immobilized Enzyme and Its Adsorption Properties for Lysozyme

A new cation exchange resin was prepared based on surface-initiated atom transfer radical polymerization (SI-ATRP) of poly (sodium 4-styrene sulfonate) (PNASS) at 30℃ for 3 h from inactivated immobilized enzymes. The cation exchange resin was characterized by Fourier transform infrared spectrometry ( FT-IR) . Lysozyme was selected as a model protein to evaluate the adsorption performance of the as-prepared cation exchange resin. The effects of protein concentration, ionic strength, organic solvent, contacting time and temperature on protein adsorption were investigated. The results showed that the cation exchange resin exhibited very good performance for the adsorption of lysozyme with a high capacity ( 240 mg/g ) at room temperature and fast speed ( within 30 min ) , which were much better than those reported in previously reported works. The adsorption isotherms could be better fitted by Langmuir model than Freundlich model and the adsorption process followed the pseudo-second-order kinetic model.