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Production of recombinant bleaching enzymes from thermophilic microorganisms in fungal hosts
Authors:Bergquist  Peter L.  Te’o  V. S. Junior  Gibbs   Moreland D.  Cziferszky  Angela C. E.  De Faria  Fabricia P.  Azevedo  Maristela O.  Nevalainen  K. M. Helena
Affiliation:(1) Department of Biological Sciences, Macquarie University, 2109 Sydney, NSW, Australia;(2) Division of Molecular Medicine, University of Auckland Medical School, 1000 Auckland, New Zealand;(3) Institute of Biochemical Technology and Microbiology, Section of Microbial Biochemistry, Technical University of Vienna, A-1040 Vienna, Austria;(4) Department de Biotecnologia, FAENQUIL, CP116, Lorena-SP, Brazil;(5) Department de Biologia Celular, IB UnB 70.910-900, Brasilia-DF, Brazil
Abstract:Cost-effective production of enzymes for industrial processes makes the appropriate selection of the host-vector expression system critical. We have developed two systems for the bulk production of bleaching enzymes from thermophiles. Kluyveromyces lactis has been developed as a secretion host employing expression vectors based on the 2μ-like plasmid pKD1 of Kluyveromyces drosophilarium. Our second system involves the filamentous fungus Trichoderma reesei. Fusion and nonfusion vectors have been constructed using the strong cellobiohydrolase 1 (cbh1) promoter. The KEX2 protease cleavage site and a 6 × HIS-tag have been incorporated to facilitate both cleavage and purification of the mature foreign proteins.
Keywords:Trichoderma reesei    Kluyveromyces lactis   thermophilic xylanases  heterologous expression  gene redesign
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