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由D型氨基酸设计自组装短肽D-EAK16构建新型三维纳米纤维支架材料
引用本文:余祖孝,陈佳,罗忠礼.由D型氨基酸设计自组装短肽D-EAK16构建新型三维纳米纤维支架材料[J].高等学校化学学报,2009,30(6):1131.
作者姓名:余祖孝  陈佳  罗忠礼
作者单位:1. 四川理工学院材料与化学工程系, 自贡 643000; 2. 重庆工商大学废油资源化技术与装备教育部工程中心生物材料实验室, 重庆 400067; 3. 四川大学华西医院, 纳米生物医学技术实验室, 成都 610065
基金项目:国家"九八五"-Ⅱ期平台项目和四川省材料腐蚀与防护重点实验室项目 
摘    要:采用D型氨基酸设计自组装短肽D-EAK16, 运用圆二色仪及原子力显微镜等仪器和细胞三维培养, 发现短肽D-EAK16在30 ℃时具有稳定的二级结构β-sheet, 在一定浓度下D-EAK16可形成由纳米纤维构成的透明水凝胶, 含水量高达99%, 可在细胞培养基(如PBS, DMEM)中形成支架. 细胞三维培养显示, 该水凝胶对细胞HO-8910和SPC-A-1的生长未见毒性. 比较D型氨基酸纳米支架和L型氨基酸纳米支架, 细胞的毒性未发现显著性差异. 采用D型氨基酸构建的自组装短肽, 可提供一个三维基质培养系统, 期望能广泛应用于生物医学工程等领域.

关 键 词:D型氨基酸  D  自组装短肽  纳米纤维  细胞支架  
收稿时间:2008-09-11

Designment of a New Self-assembling Peptide D-EAK16 with D Amino Acid into 3D Nanofiber Scaffold
YU Zu-Xiao,CHEN Jia,LUO Zhong-Li.Designment of a New Self-assembling Peptide D-EAK16 with D Amino Acid into 3D Nanofiber Scaffold[J].Chemical Research In Chinese Universities,2009,30(6):1131.
Authors:YU Zu-Xiao  CHEN Jia  LUO Zhong-Li
Institution:1. Department of Material and Chemical Engineering, Sichuan University of Science & Engineering, Zigong 643000, China; 2. Biomaterial Lab, Engineering Research Centre for Waste Oil Recovery Technology and Equipment, Ministry of Education, Chongqing Technology and Business University, Chongqing 400067, China; 3. West China Hospital, Laboratory for Nanobiomedical Technology, Sichuan University, Chengdu 610065, China
Abstract:A new generation self-assembling peptide as a research model was synthesized with D amino acid. Circular dichroism, AFM and cell 3D culture were used to study the objective model, D-EAK16. The result reveal that a stable secondary structure of D-EAK16 is β-sheet at 30 ℃. D-EAK16 can form transparent hydrogel with 99% water content, which can self-assemble to nanofibers and to the scaffold in physiological solution such as PBS and DMEM. Toxicity of the ECM(extracellular matrices) was not found when cultured the cell stains HO-8910 and SPC-A-1 in the solution of D-EAK16. Moreover, there is no significant difference to toxicity between the nano-scaffolds made of D or L amino acid. We hope these synthetic peptides can be as a 3D microenvironment of the cells(just similar to the bodies), widely use in the biomedical engineering.
Keywords:D Amino acid  D-Peptide  Self-assembling peptide  Nanofiber  Cell-scaffold  
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