Application of l-cysteine-capped nano-ZnS as a fluorescence probe for the determination of proteins |
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Authors: | Chang Qing Zhu Dan Hua Zhao Jin Long Chen Yong Xin Li Le Yu Wang Lun Wang Yun You Zhou Shu Juan Zhuo Yu Qin Wu |
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Institution: | (1) College of Chemistry and Materials Science, Anhui Normal University, 241000 Wuhu, P.R. China |
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Abstract: | Nanometer-sized l-cysteine-capped ZnS particles have been synthesized and used as a fluorescence probe to investigate the effect of proteins on fluorescent intensity. With ![Delta](/content/05p3a5fa8kuyumxa/xxlarge916.gif) =190 nm, maximum and constant synchronous fluorescence enhancement was produced at 267 nm and pH 5.12 in the presence of proteins. A highly sensitive synchronous fluorescence method for the rapid determination of proteins has been developed. Under optimum conditions, calibration graphs are linear over the range 0.03–8.0 g mL–1 for bovine serum albumin (BSA), 0.01–6.0 g mL–1 for human serum albumin (HSA), 0.05–8.0 g mL–1 for -globulin ( -G), and 0.04–4.0 g mL–1 for ovalbumin, respectively. The relative standard deviations of seven replicate measurements were 1.75% for 1.0 g mL–1 BSA, 1.90% for 1.0 g mL–1 HSA, 1.65% for 1.0 g mL–1 -G, and 2.32% for 1.0 g mL–1 ovalbumin. |
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Keywords: | l-Cysteine-capped nano-ZnS" target="_blank">l-Cysteine-capped nano-ZnS Protein Synchronous fluorescence |
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