中国明对虾精子发生过程中H4蛋白的免疫定位

以真核生物细胞核碱性蛋白H4的高度保守性为依据,利用改良的胶体金电镜免疫定位技术,研究中国明对虾精子发生过程中细胞核碱性蛋白H4的变化特征.发现从精原细胞至精母细胞的细胞质中有H4蛋白的合成,其用于该阶段细胞核染色质的构建;精细胞变态过程中,初期的早些时候有细胞质合成的H4蛋白进入前顶体囊,从初期的晚期开始,细胞核中H4蛋白向顶体囊转移,变态中期转移最强烈,晚期呈减弱趋势,最终导致成熟精子细胞核中不存在碱性蛋白H4.中国明对虾成熟精子细胞核中碱性蛋白H4的缺失是其呈非浓缩核状态的原因之一.

Immunolocalization of Basic Protein H4 During Spermatogenesis of Fenneropenaeus chinensis

Based on the extraordinary conservatism of basic protein H4 among different eucaryotic cells,the metastasis characteristics of basic protein H4 during spermatogenesis of Fenneropenaeus chinensis was studied using the improved technique of aurosol electron microscope immunolocalization.Result showed that H4 was synthesized within the cytoplasm of spermatogium and spermatocyte,and the basic protein H4 involved in the chromosomal construction;During the spermiogenesis,in the early stage of prophase,the basic protein synthesized in cytoplasm gathered in the proacrosomal vacuole.And in the late stage of prophase,H4 in the nucleus started to shift into the acrosomal vesicle.There was a lot of H4 that shifed from nucleus into the acrosomal vesicle in the metaphase,and it decreased in the anaphase.The diversion of H4 from nucleus to the acrosomal vesicle resulted in no basic protein H4 in the mature sperm nucleus,which is one of the causes of non-condensing sperm nucleus.