DNA-polymer micelles as nanoparticles with recognition ability |
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Authors: | Talom Renée Mayap Fuks Gad Kaps Leonard Oberdisse Julian Cerclier Christel Gaillard Cédric Mingotaud Christophe Gauffre Fabienne |
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Affiliation: | Université de Toulouse, CNRS, Laboratoire des IMRCP, UMR 5623, 118 route de Narbonne, 31062 Toulouse Cedex 9, France. |
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Abstract: | The Watson-Crick binding of DNA single strands is a powerful tool for the assembly of nanostructures. Our objective is to develop polymer nanoparticles equipped with DNA strands for surface-patterning applications, taking advantage of the DNA technology, in particular, recognition and reversibility. A hybrid DNA copolymer is synthesized through the conjugation of a ssDNA (22-mer) with a poly(ethylene oxide)-poly(caprolactone) diblock copolymer (PEO-b-PCl). It is shown that, in water, the PEO-b-PCl-ssDNA(22) polymer forms micelles with a PCl hydrophobic core and a hydrophilic corona made of PEO and DNA. The micelles are thoroughly characterized using electron microscopy (TEM and cryoTEM) and small-angle neutron scattering. The binding of these DNA micelles to a surface through DNA recognition is monitored using a quartz crystal microbalance and imaged by atomic force microscopy. The micelles can be released from the surface by a competitive displacement event. |
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Keywords: | block copolymers micelles molecular recognition oligonucleotides surface patterning |
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