Fluorometric determination of proteins using the terbium (III)-2-thenoyltrifluoroacetone-sodium dodecyl benzene sulfonate-protein system |
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| Authors: | Zhen Jia Jinghe Yang Xia Wu Changying Guo |
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| Affiliation: | a Key Laboratory of Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China
b Department of Chemistry, Dezhou University, Dezhou 253023, PR China |
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| Abstract: | It is found that in hexamethylene tetramine (HMTA)-HCl buffer of pH=8.00, proteins can enhance the fluorescence of terbium (III) (Tb3+)-2-thenoyltrifluoroacetone (TTA)-sodium dodecyl benzene sulfonate (SDBS) system. Based on this, a sensitive method for the determination of proteins is proposed. The experiments indicate that under the optimum conditions, the enhanced fluorescence intensity is in proportion to the concentration of proteins in the range of 4.0×10−9-7.5×10−6 g/mL for bovine serum albumin (BSA), 5.0×10−9-1.5×10−5 g/mL for human serum albumin (HSA), 1.0×10−8-7.5×10−6 g/mL for egg albumin (EA). Their detection limits (S/N=3) are 0.5, 0.8 and 2.0 ng/mL, respectively. The interaction mechanism is also studied. |
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| Keywords: | Fluorescence Protein TTA SDBS Tb3+ |
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