流动注射-石英晶体微天平中血清蛋白对扁桃酸的手性识别

采用流动注射-石英晶体微天平(FIA-QCM)和紫外光谱法研究了牛/羊血清蛋白(BSA和GSA)与扁桃酸的手性识别。 在QCM表面，BSA和GSA的浓度分别为4.9×10-12和8.8×10-12 mol/cm2。 血清蛋白与R-扁桃酸(R-MA)作用引起QCM振动频率变化值比S-扁桃酸(S-MA)大；相应紫外结合常数也比与S-MA大，说明血清蛋白对R-MA的作用强于与S-对映体。 从识别因子分析，GSA对扁桃酸的手性识别能力优于BSA。

Chiral Recognition of Mandelic Acid with Bovine and Goat Serum Albumins by Flow Injection Analysis-Quartz Crystal Microbalance

Interactions of mandelic acid(MA) and bovine/goat serum albumins(BSA/GSA) have been investigated by FIA-QCM and UV-Vis spectroscopy, respectively. Based on frequency shift of quartz crystal, surface concentrations of immobilized BSA and GSA were 4.9×10-12 and 8.8×10-12 mol/cm2, respectively. After injecting mandelic acid into FIA-QCM system, frequency of the quartz crystal decreases due to interactions between enantiomers and SA molecules and the shifts between SA and R-MA are greater than those with S-enantiomer, indicating that the interactions of SA molecules with R-MA are stronger than those with S-MA. The UV binding constants for SA with S-MA are smaller than those with R-enantiomer. Taking into account the discrimination factors(α-QCM and α-UV), GSA shows a better chiral recognition with mandelic acid than BSA.