Microcalorimetric study on expression of foreign genes in Bacillus thuringiensis

The thermogenic curves of the aerobic metabolism of the three strains of Bacillus thuringiensis B.t. A, B.t. B and B.t. C have been determined by using an LKB-2277 BioActivity Monitor. B.t. A was the host bacterium without foreign gene. B.t. B and B.t. C were constructed by transforming different foreign genes into the host B.t. A, respectively. B.t. B expressed erythromycin resistant gene, while B.t. C expressed both erythromycin resistant gene and tyrosinase gene. The heat flow rate of these strains is B.t. A> B.t. B >B.t. C. These results indicated that there is obvious interrelation between expression of foreign genes and heat flow rate of B.t. strains. This revised version was published online in August 2006 with corrections to the Cover Date.