Rosmarinic Acid and Antioxidant Enzyme Activities in <Emphasis Type="Italic">Lavandula vera</Emphasis> MM Cell Suspension Culture: A Comparative Study |
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| Authors: | Milen Georgiev Radoslav Abrashev Ekaterina Krumova Klimentina Demirevska Mladenka Ilieva Maria Angelova |
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| Institution: | (1) Department of Microbial Biosynthesis and Biotechnologies—Laboratory in Plovdiv, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Boulevard, 4002 Plovdiv, Bulgaria;(2) Department of Mycology, Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 26, 1113 Sofia, Bulgaria;(3) Department of Plant Stress Molecular Biology, Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 21, 1113 Sofia, Bulgaria |
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| Abstract: | The growth and intracellular protein content of lavender (Lavandula vera MM) cell suspension culture was followed along with some antioxidant defense system members—non-enzymatic (rosmarinic acid)
and enzymatic superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6)]. It was found that the media content and the
cultivation mode strongly influenced the production of plant defense compounds as well as the ratio between non-enzymatic
and enzymatic ones. The bioreactor culture contains about two times more rosmarinic acid, superoxide dismutase, and catalase
compared to the shake-flask cultivation. These findings are discussed with respect to the relative stress levels and plant
antioxidant orchestra system. It was concluded that investigated defense system components (enzymatic and non-enzymatic) were
closely associated in a complex balance. The three isoenzyme forms of SOD (Cu/ZnSOD, FeSOD, and MnSOD) in the cells of Lavandula vera were revealed by polyacrylamide gel electrophoresis analysis, and the FeSOD isoform exhibited highest activity. |
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