The dependence of DNA luminescence on excitation wavelength in the UV/VUV region |
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Authors: | S Basu RB Cundall MW Jones GO Phillips |
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Institution: | Department of Chemistry and Applied Chemistry, University of Salford, Salford M5 4WT, UK;North East Wales Institute, Deeside, Clwyd, UK |
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Abstract: | Luminescence emission and excitation spectra have been obtained for DNA films at 77 K under vacuum ultraviolet excitation (150–280 nm). The emission spectra, which cover the wavelength range 310 to 490 nm, consists of two components, a short-lived component around 350 nm which is attributed to fluorescence and a longer-lived component around 410 nm believed to be phosphorescence. The excitation spectra, as functions of emission wavelength, are similar in profile with a fairly broad peak around 9240–260 nm) with a shoulder around 200 nm followed by a gradual but constant decrease into the vacuum ultraviolet region of the spectrum. No evidence of autoionization was seen in the region investigated. |
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