Elemental distribution by synchrotron X-ray microfluorescence of prostate 3D cell culture |
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| Authors: | K.M.J. Rocha R.G. Leitão E.G. Oliveira-Barros M.A. Oliveira C.G.L. Canellas M.J. Anjos L.E. Nasciutti R.T. Lopes |
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| Affiliation: | 1. Nuclear Instrumentation Laboratory, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;2. Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;3. Physics Institute, Stated University of Rio de Janeiro, Rio de Janeiro, Brazil;4. Nuclear Instrumentation Laboratory, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Physics Institute, Stated University of Rio de Janeiro, Rio de Janeiro, Brazil |
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| Abstract: | Prostate cancer is a highly prevalent disease and ranks second among malignant neoplasms that affect men around the world, behind lung cancer alone. Trace elements are very important and are involved in many cellular processes. The X-ray microfluorescence technique is an advanced tool of high spatial resolution, sensitivity, multielemental analysis, and nondestructiveness for trace element study. This study aimed to investigate the elemental distribution in spheroids obtained through the following human prostate cell lines using synchrotron X-ray microfluorescence: tumor cell line androgen independent (DU145), tumor cell line androgen dependent (LNCaP), and normal cell line (RWPE-1). The measurements were performed with a standard geometry of 45° of incidence, excited by a white beam using a pixel of 25 μm and an acquisition time of 300 ms/pixel at the X-ray fluorescence beamline at the Synchrotron Light National Laboratory (Campinas, Brazil). The synchrotron X-ray microfluorescence results showed differences between groups in all elements analyzed and suggested that further studies should be performed to understand the relationship of these trace elements with the progression and development of the disease. |
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