Mannose-Modified Multi-Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells |
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Authors: | Zhipeng Dong Qiyan Wang Ming Huo Nanxia Zhang Bingxia Li Hongmei Li Yisong Xu Meng Chen Prof Hao Hong Prof Yue Wang |
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Institution: | 1. Key Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing 211198, Jiangsu Province China;2. Nanjing Foreign Language School, NO.30 East Beijing Road, Nanjing, 210029 China;3. Center for Molecular Imaging, Department of Radiology, University of Michigan, Ann Arbor, Michigan, 48109-2200 United States |
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Abstract: | Dendritic cells (DCs) based cancer immunotherapy is largely dependent on adequate antigen delivery and efficient induction of DCs maturation to produce sufficient antigen presentation and ultimately lead to substantial activation of tumor-specific CD8+ T cells. Carbon nanotubes (CNTs) have attracted great attention in biomedicine because of their unique physicochemical properties. In order to effectively deliver tumor antigens to DCs and trigger a strong anti-tumor immune response, herein, a specific DCs target delivery system was assembled by using multi-walled carbon nanotubes modified with mannose which can specifically bind to the mannose receptor on DCs membrane. Ovalbumin (OVA) as a model antigen, could be adsorbed on the surface of mannose modified multi-walled carbon nanotubes (Man-MWCNTs) with a large drug loading content. This nanotube-antigen complex showed low cytotoxicity to DCs and was efficiently engulfed by DCs to induce DCs maturation and cytokine release in vitro, indicating that it could be a potent antigen-adjuvant nanovector of efficient antigen delivery for therapeutic purpose. |
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Keywords: | dendritic cells carbon nanotubes antigen presentation immunotherapy drug delivery |
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