Characterization of different RP-HPLC columns by a gradient elution technique |
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| Authors: | Á Sándi Á Bede L Szepesy G Rippel |
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| Institution: | (1) Department of Chemical Technology, Technical University of Budapest, Budafoki u.8., 1521 Budapest, Hungary;(2) Department of Agricultural Chemical Technology, Technical University of Budapest, Budafoki u.8., 1521 Budapest, Hungary |
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| Abstract: | Summary The literature contains no unified testing procedure for evaluation and characterization of reversed-phase (RP) columns of
different pore size (surface area) and containing different types of ligand. In our laboratory a testing procedure has been
developed using gradient elution under standardized conditions independent of column dimensions and applicable to both narrow-pore
and wide-pore columns. Six wide-pore and four narrow-pore columns were investigated and compared. The test solutes were selected
to cover a wide range of chemical properties. The evaluation and characterization of the columns was performed on the basis
ofk
app retention factors. Principal components analysis (PCA) was performed to reveal similarities and differences among the columns
and test substances. The factors obtained characterize the columns according to the extent of various interactions such as
hydrophobicity, HB (hydrogen bond)-donor acidity and HB-acceptor basicity.
Presented at the 21st ISC held in Stuttgart, Germany, 15th–20th September, 1996 |
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| Keywords: | RP-HPLC columns of different pore size and ligands Gradient elution Multiparametric characterization Chemometric characterization Principal components analysis |
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