| meso—四（对—羟基苯基）卟啉与脱氧核糖核酸的作用及其分析应用 |
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| 引用本文： | 黄承志,李克安.meso—四（对—羟基苯基）卟啉与脱氧核糖核酸的作用及其分析应用[J].分析化学,1997,25(9):1052-1056. |
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| 作者姓名： | 黄承志 李克安 |
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| 作者单位： | 西南师范大学环境化学研究所!重庆630715(黄承志)，北京大学化学系!北京100871(李克安，童沈阳) |
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| 基金项目： | 国家自然科学基金，国家教育委员会博士点基金资助课题。 |
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| 摘 要： | 报道了非离子型卟啉ｍｅｓｏ－四卟啉与脱氧核糖核酸的作用。在ＰＨ４．９－５．４范围内，ＤＮＡ能与聚合态的ＴＨＰ作用，产生不规则的电子吸收光谱。但如果体系中含有３０％（Ｖ／Ｖ）乙醇，ＤＮＡ与ＴＨＰＰ作用产生４５９．０ｎｍ和７００．０ｎｍ两个新峰。
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| 关 键 词： | 卟啉 DNA THPP 分光光度法 |
Interaction of meso -Tetrakis (4-hydroxyphenyl)porphine With Deoxyribonucleic Acids and Analytical Applications |
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| Huang Chengzhi.Interaction of meso -Tetrakis (4-hydroxyphenyl)porphine With Deoxyribonucleic Acids and Analytical Applications[J].Chinese Journal of Analytical Chemistry,1997,25(9):1052-1056. |
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| Authors: | Huang Chengzhi |
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| Abstract: | In the present paper the interactions of a nonionic porphyrin-meso-tetrakis(4- hydroxyphenyl)porphine with deoxyribonucleic acids (DNAs) are reported. DNAs can inter- act with the aggregated species of the titled porphyrin in the pH range of 4. 9-5. 4 and give irregular electronic absorption spectra. Two new absorption bands with the peaks located at 459. 0 nm and 700. 0 nm can be observed if 30% (V/V) ethanol exists in the system. Based on the absorption band at 459. 0 nm, calf thymus DNA ranging 0. 2- 5. 0 mg/L and fish sperm DNA ranging 0. 2-6. 0 mg/L can be determined with good reproducibi1ity. The limit of determination for calf thymus DNA and fish sperm DNA is 0. 02 mg/L and 0. 03 mg/L re- spectively. No interference was found from more than l0 fold RNA. Determinations of DNA in four formulated samples are satisfactory. |
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| Keywords: | meso-Tetrakis (4-hydroxyphenyl ) porphine calf thymus deoxyribonucleic acid fish sperm deoxyribonucleic acid spectrophotometry |
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