Radio high-performance liquid chromatographic determination of 14C-labelled LF 2-0254, A 1,4-dihydropyridine calcium antagonist, in rat and dog plasma using off-line liquid scintillation counting

LF 2-0254 is a 1,4-dihydropyridine calcium antagonist with a slow onset of action. The pharmacokinetics of 14C]LF 2-0254 were studied in rats and dogs. A sensitive high-performance liquid chromatographic method using liquid scintillation counting was developed for the quantitation of labelled LF 2-0254 in plasma. The peak height of the internal standard in the chromatogram was measured by UV detection and the mobile phase containing the chromatographic peak of 14C]LF 2-0254 was collected and counted for radioactivity. The concentration of labelled drug in the plasma was then determined using a calibration graph constructed from the determination of 14C]LF 2-0254 of known specific activities. The limit of determination was dependent on the specific activity of the drug administered. This method permits the measurement of the radioactive drug in biological fluids.