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Long-term quantitative phase-contrast imaging of living cells by digital holographic microscopy |
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| Authors: | Liu S Pan F Wang Z Wang F Rong L Shang P Xiao W |
| Institution: | 1.School of Instrument Science and Optoelectronics Engineering, Beihang University, Beijing, 100191, China ;2.Key Laboratory for Space Biosciences and Biotechnology, Faculty of Life Sciences, Northwestern Polytechnical University, Xi’an, 710072, China ; |
| Abstract: | The dynamic analysis of biological living samples is one of the particular interests in life sciences. An improved digital holographic microscope for long-term quantitative phase-contrast imaging of living cells is presented in this paper. The optical configuration is optimized in the form of a free-space-fiber hybrid system which promotes the flexibility of imaging in complex or semi-enclosed experimental environment. Aberrations compensation is implemented taking into account the additional phase aberration induced by liquid culture medium in long-term observation. The proposed approach is applied to investigate living samples of MC3T3-E1 and MLO-Y4 cells. The experimental results demonstrate its availability in the analysis of cellular changes. |
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