Determination of vitamin B12 in pharmaceutical preparations by a highly sensitive fluorimetric method |
| |
Authors: | Hua-Bin Li Feng Chen |
| |
Affiliation: | (1) Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong e-mail: sfchen@hkusua.hku.hk, CN |
| |
Abstract: | A fluorimetric method for the determination of vitamin B12 has been developed. The fluorescence emission was measured at λex/λem275/305 nm in phosphate buffer solution (pH 7.0), and the experimental variables and possible interference were studied. The linear calibration range was 1.000 ng/mL to 100.0 ng/mL with a correlation coefficient of 0.9994 and a detection limit of 0.1 ng/mL. The method is rapid, simple and highly sensitive. It was used to determine vitamin B12 in pharmaceutical preparations. The recovery was 96%–98% and the relative standard deviation was in the range of 1.8%–2.7%. The results agreed with those obtained by spectrophotometry. Received: 17 July 2000 / Revised: 4 September 2000 / Accepted: 7 September 2000 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|