Antimicrobial Photodynamic Efficiency of Novel Cationic Porphyrins towards Periodontal Gram‐positive and Gram‐negative Pathogenic Bacteria |
| |
Authors: | Chandra Sekhar Prasanth Suneesh C Karunakaran Albish K Paul Vesselin Kussovski Vanya Mantareva Danaboyina Ramaiah Leslie Selvaraj Ivan Angelov Latchezar Avramov Krishnankutty Nandakumar Narayanan Subhash |
| |
Institution: | 1. Biophotonics Laboratory, Centre for Earth Science Studies, , Trivandrum, India;2. Photosciences and Photonics, Chemical Sciences and Technology Division, CSIR‐ National Institute for Interdisciplinary Science and Technology, , Trivandrum, India;3. The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, , Sofia, Bulgaria;4. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, , Sofia, Bulgaria;5. Microbiology Division, Doctors Diagnostic Research Centre, , Trivandrum, India;6. Institute of Electronics, Bulgarian Academy of Sciences, , Sofia, Bulgaria;7. Department of Periodontics, Azeezia Dental College, , Kollam, India |
| |
Abstract: | The Gram‐negative Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are major causative agents of aggressive periodontal disease. Due to increase in the number of antibiotic‐resistant bacteria, antimicrobial Photodynamic therapy (aPDT) seems to be a plausible alternative. In this work, photosensitization was performed on Gram‐positive and Gram‐negative bacteria in pure culture using new‐age cationic porphyrins, namely mesoimidazolium‐substituted porphyrin derivative ( ImP ) and pyridinium‐substituted porphyrin derivative ( PyP ). The photophysical properties of both the sensitizers including absorption, fluorescence emission, quantum yields of the triplet excited states and singlet oxygen generation efficiencies were evaluated in the context of aPDT application. The studied porphyrins exhibited high ability to accumulate into bacterial cells with complete penetration into early stage biofilms. As compared with ImP, PyP was found to be more effective for photoinactivation of bacterial strains associated with periodontitis, without any signs of dark toxicity, owing to its high photocytotoxicity. |
| |
Keywords: | |
|
|