Purification of drugs from biological fluids by counter-current chromatography |
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| Authors: | Jill E Hochlowski Jeffrey Y PanPhilip A Searle Wayne R BuckStephen G Spanton |
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| Institution: | Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064, USA |
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| Abstract: | Experiments were performed to demonstrate the potential of counter-current chromatography (CCC) for the isolation of drugs and their metabolites from biological matrices relevant to the metabolism studies of pharmaceutical research. Examples of typical drugs are spiked into biological media ex vivo to provide test samples for analysis. A mass spectrometer hyphenated to a CCC allows for the detection of small molecule drugs within the matrix through selected ion monitoring, and fraction collection can provide material for further structural elucidation by NMR. |
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| Keywords: | Counter-current chromatography Pharmaceutical discovery Drugs Bile Serum Metabolites |
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