Aqueous chromatographic system for the quantification of propofol in biological fluids using a temperature-responsive polymer modified stationary phase |
| |
Authors: | Tadashi Nishio Rie Suzuki Yuko Tsukada Hideko Kanazawa Teruo Okano Takako Miyabe-Nishiwaki |
| |
Affiliation: | 1. Faculty of Pharmacy, Keio University, 1-5-30, Shibakoen, Minato-ku, Tokyo 105-8512, Japan;2. Institute of Advanced Biomedical Engineering and Science, Tokyo Women''s Medical University, 8-1 Kawadacho, Shinjyuku-ku, Tokyo 162-8666, Japan;3. Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan |
| |
Abstract: | A new method for the quantitative analysis of monkey serum propofol, which is widely used as an anaesthetic agent, was developed by utilizing a temperature-responsive polymer of N-isopropylacrylamide (NIPAAm) and butyl methacrylate (BMA) as the stationary phase of HPLC–fluorescence detection. This poly(NIPAAm-co-BMA) copolymer undergoes a reversible phase transition from a hydrophilic to a hydrophobic microstructure when triggered by change in the temperature. Also this chromatographic system is possible to separate the analytes by using only water as a mobile phase. A pretreatment of the serum (80 μL) was only solid-phase extraction, and the recovery rate of propofol and internal standard was more than 77%, respectively. This method covered the calibration range from 0.5 μg/mL to 10 μg/mL and allowed a reproducible quantification of the serum propofol in administrated monkey serum. The intra- and inter-assay relative standard deviations were less than 14.1%. In addition, there was good relationship of the quantification values between the developed method and the widely used reversed-phase HPLC method. Our developed method has proven to be useful for a simple analysis of propofol in clinical practice, because the avoidance of complicated mobile phase preparation was possible, and only temperature changing could regulate the retention time of the analyte. In addition, by using water instead of fossil fuel, it is the ideal analytical method according to green chemistry. |
| |
Keywords: | Poly(N-isopropylacrylamide) Temperature-responsive chromatography HPLC&ndash fluorescence detection Propofol Clinical practice Green chemistry |
本文献已被 ScienceDirect 等数据库收录! |
|