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Isolation of the new minor constituents dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography |
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| Authors: | Krystyna Skalicka-Woźniak Tomasz Mroczek Ian Garrard Kazimierz Głowniak |
| Affiliation: | 1. Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland;2. Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge UB8 3PH, UK |
| Abstract: | A preparative high-speed counter-current chromatography (HSCCC) method was successfully used for isolation of two new minor compounds – alsaticol and alsaticocoumarin A. A two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (1:1:1:1) was developed. Compounds were obtained from the dichloromethane extract of Peucedanum alsaticum fruits and their identification was performed with NMR and MS methods. Optimized HSCCC offers a rapid method of obtaining new natural compounds. |
| Keywords: | High-speed counter-current chromatography Peucedanum alsaticum Coumarin Pyranochromone Preparative chromatography |
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