Theoretical advantages and drawbacks of on-line,multidimensional liquid chromatography using multiple columns operated in parallel |
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Authors: | Jacob N Fairchild Krisztián Horváth Georges Guiochon |
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Institution: | 1. Department of Chemistry, University of Tennessee, Knoxville, TN 37996–1600, USA;2. Department of Analytical Chemistry, University of Pannonia, P.O. Box 158, Veszprém H-8200, Hungary |
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Abstract: | The theoretical advantages and drawbacks of using a multiple-, parallel column approach in on-line multidimensional liquid chromatography systems were investigated. Much time or peak capacity can be gained with the use of multiple parallel columns at the second-dimension while the aggregate time of separation increases only by the increment of the gradient time of the second-dimension. Multidimensional chromatographic systems are now used to perform many tasks ranging from routine, fast analyses to specialized, arduous separations. In this work, we focus on the advantages of a multiple, parallel columns approach to on-line multidimensional liquid chromatography systems. Calculations of the achievable peak capacities were made as functions of the number of columns operated in parallel. Increasing the number of second-dimension columns from one to two or three causes the largest increase in peak capacity with only a slight increase of aggregate time. We also present some practical aspects to consider when attempting multidimensional separations with multiple columns operated in parallel. |
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Keywords: | Two-dimensional liquid chromatography Multidimensional chromatography Parallel columns |
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