Selective determination method for measurement of nitrite and nitrate in water samples using high-performance liquid chromatography with post-column photochemical reaction and chemiluminescence detection |
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Authors: | Hitoshi Kodamatani Shigeo Yamazaki Keiitsu Saito Takashi Tomiyasu Yu Komatsu |
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Institution: | 1. Department of Earth and Environmental Science, Faculty of Science, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan;2. Department of Applied Science, Okayama University of Science, 1-1 Ridaicho, Okayama 700-0005, Japan;3. Division of Human Environment, Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada, Kobe 657-8501, Japan;4. Environmental Research Institute, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan 924-0838, Japan |
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Abstract: | A simple, sensitive and selective method for the simultaneous determination of nitrite and nitrate in water samples has been developed. The method is based on ion-exchange separation, online photochemical reaction, and luminol chemiluminescence detection. The separation of nitrite and nitrate was achieved using an anion-exchange column with a 20 mM borate buffer (pH 10.0). After the separation, these ions were converted to peroxynitrite by online UV irradiation using a low-pressure mercury lamp and then mixed with a luminol solution prepared with carbonate buffer (pH 10.0). The calibration graphs of the nitrite and nitrate were linear in the range from 2.0 × 10−9 to 2.5 × 10−6 M and 2.0 × 10−8 to 2.5 × 10−5 M, respectively. Since the sensitivity of nitrite was about 10 times higher than that of nitrate, the simultaneous determination of nitrite and nitrate in the water samples could be efficiently achieved. This method was successfully applied to various water samples – river water, pond water, rain water, commercial mineral water, and tap water – with only filtration and dilution steps. |
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Keywords: | Nitrite Nitrate Peroxynitrite Photochemical reaction Luminol Chemiluminescence |
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