Improvement of the high-speed log D assay using an injection marker for the water plug aspiration/injection method |
| |
Authors: | Ikuko Nishimura Atsushi HiranoTaro Yamashita Takehiro Fukami |
| |
Affiliation: | Tsukuba Research Institute, Banyu Pharmaceutical Co., Ltd., Ohkubo 3, Tsukuba, Ibaraki 300-2611, Japan |
| |
Abstract: | An improved method for the high-speed assay for log D (HSLogD) employing an injection marker is described. The critical process of HSLogD is automated sampling of the water phase, which prevents contamination from the octanol phase. However, deployment of HSLogD sometimes produces incorrect and inaccurate values compared with data from closely related methods due to low solubility of compounds or instrument problems. Therefore, careful manual inspection of data is needed to confirm the results obtained by this method. To solve this problem, compounds were screened for suitability as injection markers and 2-(4-methoxyphenoxy)-4,8-dimethylquinoline was the most suitable compound examined. Introduction of the injection marker into the process successfully eliminated the need for the tedious and time-consuming manual inspection process. |
| |
Keywords: | Log D measurements High-speed log D Lipophilicity Injection marker |
本文献已被 ScienceDirect 等数据库收录! |
|