Development of vial wall sorptive extraction and its application to determination of progesterone in human serum |
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Authors: | Migaku Kawaguchi Shin-ichiro Fujii Nobuyasu Itoh Rie Ito Hiroyuki Nakazawa Akiko Takatsu |
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Affiliation: | 1. National Institute of Advanced Industrial Science and Technology (AIST), National Metrology Institute of Japan (NMIJ), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan;2. Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan |
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Abstract: | A novel sample preparation method, vial wall sorptive extraction (VWSE), which uses a vial whose internal wall is coated with polydimethylsiloxane (PDMS), was developed. The method was applied to the determination of progesterone in human serum sample. Human serum sample (0.5 mL) spiked with progesterone-13C2 was pipetted into the VWSE device and vortex mixing was performed for 30 min. Then, the serum sample was removed and the vial rinsed with purified water. Fifty microliter of methanol as liquid desorption (LD) solvent was pipetted into the VWSE device and vortex mixing was performed for 10 min. Then, the extract was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The correlation coefficient (r) of the calibration curve over the concentration range of 0.5–200 ng mL−1 was 0.999. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.1 and 0.5 ng mL−1, respectively. The relative recoveries were 97.9% (RSD: 4.4%, n = 6) and 102.8% (RSD: 1.1%, n = 6) for progesterone spiked at 5 and 50 ng mL−1, respectively. This simple, accurate, sensitive, and selective analytical method is applicable to the trace analysis of a minute amount of sample. |
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Keywords: | Vial wall sorptive extraction (VWSE) Miniaturization Progesterone Liquid chromatography-tandem mass spectrometry (LC-MS/MS) |
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