Optimization of a heart-cutting multidimensional gas chromatography-based method for the assessment of enantiomeric fractions of o,p′-DDT in environmental samples

The enantiomeric fractions present in soil samples may provide information useful in distinguishing recent inputs of DDT from past DDT pollution. In this study, a chromatographic procedure for the determination of the enantiomeric fractions of o,p′-DDT based on heart-cutting multidimensional gas chromatography was developed. The optimization carried out achieved low ratios of DDT degradation (<15%) in the chromatographic system. High selectivity and sensitivity in the detection of the target compounds, with a limit of detection as low as 2.1 pg μL⁻¹, was reached. In addition, high degrees of repeatability (RSD < 2.0%) and reproducibility (RSD < 3.2%) were obtained for the enantiomeric fractions measured in analytical standards and soil samples.