Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography |
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| Authors: | Dalene de Beer Gerold Jerz Elizabeth Joubert Victor Wray Peter Winterhalter |
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| Institution: | 1. Post-Harvest and Wine Technology Division, ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa;2. Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstrasse 20, D-38106 Braunschweig, Germany;3. Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602 Stellenbosch, South Africa;4. Department of Structural Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany |
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| Abstract: | Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid–liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid–liquid partitioning with ethyl acetate–methanol–water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether–n-butanol–acetonitrile–water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC–high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy. |
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| Keywords: | Counter-current chromatography Cyclopia subternata Fabaceae Isomangiferin Mangiferin Xanthones NMR spectroscopy |
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