Separation of proteins using a novel two-depth miniaturized free-flow electrophoresis device with multiple outlet fractionation channels |
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Authors: | Marco Becker Ulrich MarggrafDirk Janasek |
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Affiliation: | ISAS – Institute for Analytical Sciences, Bunsen-Kirchhoff-Strasse 11, 44139 Dortmund, Germany |
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Abstract: | A novel free-flow electrophoresis glass chip design with two-depth etched structures for the separation and fractionation of proteins is presented. The microfluidic structures etched in two depths enhance the flow characteristics inside the miniaturized device. A novel nine-port outlet interface enables the fractionation of the separated analytes. The separation and focussing of a protein sample mixture demonstrated the ability of the new chip. |
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Keywords: | Microfluidic Free-flow electrophoresis Microfabrication |
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