Influence of ligand density on antibody binding capacity of cation-exchange adsorbents |
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Authors: | Katarzyna Wrzosek Michal GrambličkaMilan Polakovič |
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Affiliation: | Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia |
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Abstract: | Adsorption properties of a set of polymethacrylate-based cation exchangers designed for purification of monoclonal antibodies were investigated. The materials differed significantly in the density of sulphoisobutyl ligand groups. The ligand density had a pronounced effect on the static adsorption capacity of a polyclonal human immunoglobulin G. An optimal ligand density was observed at any pH and NaCl concentration tested when sharp optima were observed at low pH and ionic strength values. This was caused by effective clogging of pore mouth at high ligand densities. An anomalous effect of ionic strength was observed for the adsorbents with the high ligand density when the adsorption capacity increased with the addition of NaCl at low pH. |
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Keywords: | Cation-exchange chromatography Monoclonal antibody Adsorption capacity Ligand density Ionic strength pH influence |
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