Separation of amino acids and amines by capillary electrophoresis using poly(ethylene oxide) solution containing cetyltrimethylammonium bromide |
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Authors: | Chuan-Kuo Chen Kung-Tien Liu Tai-Chia Chiu Huan-Tsung Chang |
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Institution: | 1. Department of Chemistry, National Taiwan University, Taipei, Taiwan;2. Chemical Analysis Division, Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan, Taiwan;3. Department of Applied Science, National Taitung University, Taitung, Taiwan |
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Abstract: | We describe simultaneous analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-amino acid and amine derivatives by capillary electrophoresis in conjunction with light-emitting diode-induced fluorescence (LEDIF) detection using poly(ethylene oxide) (PEO) containing cetyltrimethylammonium bromide (CTAB). In the presence of CTAB and acetonitrile (ACN), adsorption of PEO on the capillary wall is suppressed, leading to generation of a fast and reproducible electroosmotic flow (EOF). In order to optimize separation resolution and speed, 100 mM Tris–borate solution (pH 7.0) containing 20 mM CTAB and 25% ACN was used to fill the capillary and to prepare 1.2% PEO that entered the capillary via EOF. The analysis of 14 NDA-amino acid and -amine derivatives by this approach is rapid (< 4 min), efficient ((0.9–6.4) × 105 theoretical plates), and sensitive (the LODs (S/N = 3) range from 9.5 to 50.5 nM). The RSD values (n = 5) of the migration times and peak heights of the analytes for the intraday analysis are less than 1.5 and 1.2%, respectively. We have validated the practicality of this approach by quantitative determination of 10 amino acids and amines in a beer samples within 4 min. |
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Keywords: | Acetonitrile Amines Amino acids Cetyltrimethylammonium bromide Light-emitting diode-induced fluorescence detection Micellar electrokinetic chromatography Poly(ethylene oxide) |
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