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Separation of amino acids and amines by capillary electrophoresis using poly(ethylene oxide) solution containing cetyltrimethylammonium bromide
Authors:Chuan-Kuo Chen  Kung-Tien Liu  Tai-Chia Chiu  Huan-Tsung Chang
Institution:1. Department of Chemistry, National Taiwan University, Taipei, Taiwan;2. Chemical Analysis Division, Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan, Taiwan;3. Department of Applied Science, National Taitung University, Taitung, Taiwan
Abstract:We describe simultaneous analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-amino acid and amine derivatives by capillary electrophoresis in conjunction with light-emitting diode-induced fluorescence (LEDIF) detection using poly(ethylene oxide) (PEO) containing cetyltrimethylammonium bromide (CTAB). In the presence of CTAB and acetonitrile (ACN), adsorption of PEO on the capillary wall is suppressed, leading to generation of a fast and reproducible electroosmotic flow (EOF). In order to optimize separation resolution and speed, 100 mM Tris–borate solution (pH 7.0) containing 20 mM CTAB and 25% ACN was used to fill the capillary and to prepare 1.2% PEO that entered the capillary via EOF. The analysis of 14 NDA-amino acid and -amine derivatives by this approach is rapid (< 4 min), efficient ((0.9–6.4) × 105 theoretical plates), and sensitive (the LODs (S/N = 3) range from 9.5 to 50.5 nM). The RSD values (n = 5) of the migration times and peak heights of the analytes for the intraday analysis are less than 1.5 and 1.2%, respectively. We have validated the practicality of this approach by quantitative determination of 10 amino acids and amines in a beer samples within 4 min.
Keywords:Acetonitrile  Amines  Amino acids  Cetyltrimethylammonium bromide  Light-emitting diode-induced fluorescence detection  Micellar electrokinetic chromatography  Poly(ethylene oxide)
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