Using subcritical/supercritical fluid chromatography to separate acidic,basic, and neutral compounds over an ionic liquid-functionalized stationary phase |
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Authors: | Fu-Min Chou Wei-Ting WangGuor-Tzo Wei |
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Institution: | Department of Chemistry and Biochemistry, National Chung Cheng University, Min-Hsiung, Chia-Yi 621, Taiwan |
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Abstract: | We packed an ionic liquid (IL)-functionalized stationary phase – based on 1-octyl-3-propylimidazolium chloride covalently bounded to silica gel – into a 3.2 mm × 250 mm column for the simultaneous separation of acidic, basic, and neutral compounds using carbon dioxide subcritical/supercritical fluid chromatography (SFC), and examined the effects of the pressure, temperature, co-solvents, and additives on the retention behavior of the analytes. The model compounds tested for SFC separation are acetaminophen, metoprolol, fenoprofen, ibuprofen, naphthalene, and testosterone. The data indicate that hydrogen-bonding and hydrophobic interactions between the analytes and the IL-modified stationary phase seem to involve in the separation process. Simultaneous separation of acidic, basic, and neutral compounds via SFC was successful at a co-solvent content of 20% MeOH, a pressure of 110 bar, and a column temperature of 35 °C. The relative standard deviations of the retention times and peak areas at 50 ppm were all less than 4 and 8% (n = 6), respectively. |
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Keywords: | Ionic liquid 1-Octyl-3-propylimidazolium chloride Stationary phase Supercritical fluid chromatography |
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