| 大孔吸附树脂LX-20从发酵液中分离纯化维生素B_(12) |
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| 引用本文： | 张智聪,郑珩,顾觉奋. 大孔吸附树脂LX-20从发酵液中分离纯化维生素B_(12)[J]. 离子交换与吸附, 2011, 27(4) |
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| 作者姓名： | 张智聪 郑珩 顾觉奋 |
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| 作者单位： | 中国药科大学生命科学与技术学院,南京,210009 |
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| 摘 要： | 对从脱氮假单胞菌发酵液中分离纯化维生素B12的方法进行了研究。通过对11种弱极性大孔树脂进行筛选,确定LX-20为最适合树脂;对饱和树脂进行静态洗脱和动态洗脱,确定75%甲醇水溶液为洗脱剂,加入醋酸有利洗脱。利用薄层法对粗品进行精制。最终建立工艺路线为发酵液预处理后,LX-20吸附饱和,含醋酸的75%甲醇洗脱,经适当放大后结果仍好。此法洗脱高峰集中,产品纯度达90%,回收率可达98%以上,所得精品经HPLC检测纯度为97.8%。
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| 关 键 词： | 大孔吸附树脂LX-20 维生素B12 发酵液 分离纯化 |
EXTRACTION AND PURIFICATION OF VITAMIN B_(12) IN FERMENTATION BY MACRORETICULAR LX-20 |
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| ZHANG Zhicong,ZHENG Heng,GU Juefen. EXTRACTION AND PURIFICATION OF VITAMIN B_(12) IN FERMENTATION BY MACRORETICULAR LX-20[J]. Ion Exchange and Adsorption, 2011, 27(4) |
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| Authors: | ZHANG Zhicong ZHENG Heng GU Juefen |
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| Affiliation: | ZHANG Zhicong ZHENG Heng GU Juefen Institute of Life Science and Technology,China Pharmaceutical University Nanjing 210009,China |
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| Abstract: | To extract and purify vitamin B12 from the fermentation broth of Pseudomonas denitrificans,LX-20 was determined as the optimum resin among 11 species of macroreticular resins with large aperture and weakly polar screened;75% methanol solution was determined as the most suitable eluting agent by static and dynamic elution,and acetic acid was beneficial to elution.TLC was used to refine the crude product.The technology route that the pretreated fermentation was adsorbed with LX-20,and then eluted with acetic ... |
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| Keywords: | Vitamin B12 Fermentation broth Extraction and purification Macroreticular resin LX-20 |
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