| 壳聚糖/聚丙烯酸纳米粒子对蛋白质的吸附行为 |
| |
| 引用本文： | 赵海利,李从欣,郭天瑛.壳聚糖/聚丙烯酸纳米粒子对蛋白质的吸附行为[J].离子交换与吸附,2011,27(5). |
| |
| 作者姓名： | 赵海利 李从欣 郭天瑛 |
| |
| 作者单位： | 功能高分子材料教育部重点实验室南开大学高分子化学研究所,天津,300071 |
| |
| 基金项目： | 国家自然科学基金(20874052); 纤维材料改性国家重点实验室基金(东华大学)(LK0808) |
| |
| 摘 要： | 以马来酸酐改性的壳聚糖(MAH-chitosan)和丙烯酸(AA)为单体,采用反相微乳液聚合法制备了AA含量分别为77%(AA-Cs)和29%(Cs-AA)壳聚糖/聚丙烯酸复合纳米粒子。TEM结果表明,该复合纳米粒子平均粒径为125~150nm,进而研究了其对牛血红蛋白(Hb)、牛血清白蛋白(BSA)和溶菌酶(Ly)吸附和脱附行为。AA-Cs对各种蛋白的吸附量均较高;而Cs-AA对3种蛋白的吸附则具有一定选择性。AA-Cs对等电点低于脱附pH值的Hb的脱附量较大。Cs-AA粒子在较低pH值(pH=3.4)时对吸附有利,但在高pH值(pH=6.6)进行吸附时,对脱附更为有利。
|
| 关 键 词： | 反相微乳液聚合 壳聚糖纳米粒子 吸附与脱附 蛋白质 |
PREPARATION OF CHITOSAN/POLYACRYLIC ACID NANOPARTICLES AND THE ADSORPTION BEHAVIOR TO PROTEINS |
| |
| ZHAO Haili,LI Congxin,GUO Tianying.PREPARATION OF CHITOSAN/POLYACRYLIC ACID NANOPARTICLES AND THE ADSORPTION BEHAVIOR TO PROTEINS[J].Ion Exchange and Adsorption,2011,27(5). |
| |
| Authors: | ZHAO Haili LI Congxin GUO Tianying |
| |
| Institution: | ZHAO Haili LI Congxin GUO Tianying Key Laboratory of Functional Polymer Materials,Ministry of Education Institute of Polymer Chemistry,Nankai University,Tianjin 300071,China |
| |
| Abstract: | In this work,chitosan-acrylic acid(AA) nanoparticles were prepared by inverse microemulsion polymerization technique with maleic anhydride-modified chitosan and acrylic acid as monomers.The AA-Cs(with AA 77%) and Cs-AA(with AA 29%) nanoparticles have been prepared by changing the ratios of chitosan and AA.The results from the TEM graphs showed that the average size of the particles was about 125~150nm.The adsorption behaviors were measured with hemoglobin(Hb),bovine albumin(BSA) and lysozyme(Ly) as model pr... |
| |
| Keywords: | Inverse microemulsion polymerization Chitosan Nanoparticles Adsorption and desorption Protein |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
