ACETONE-SENSITIZED PHOTOINACTIVATION OF TRANSFORMING DNA |
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| Authors: | F W Pons H D Mennigmann |
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| Institution: | Arbeitsgruppe Mikrobengenetik im Fachbereich Biologie, Universität, Frankfurt/M., Germany |
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| Abstract: | Abstract— Irradiation of Haemophilus influenzae transforming DNA with 313 nm radiation in the presence of acetone leads to inactivation of transforming activity. Some of the lesions produced are substrate for photoreactivating enzyme and the dark-repair mechanism. In addition, other lesions are produced which, if their production is not prevented by the presence of EDTA during irradiation, render the DNA less photoreactivable. The possibility is discussed that, among others, single-strand breaks are responsible for this loss in photoreactivability. |
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