高效液相色谱法测定染发剂中的22种染料成分

建立了染发剂中22种染料成分的高效液相色谱测定方法。使用Discovery RP-Amide C16柱(250 mm×4.6 mm,5 μm),以乙腈-0.025 mol/L磷酸盐缓冲液(pH 6.0,含0.1%的庚烷磺酸钠离子对试剂)为流动相,流速为1.0 mL/min;使用二极管阵列检测器(DAD),检测波长为260 nm和280 nm,柱温为25 ℃。该方法除了对低浓度的甲苯-2,5-二胺硫酸盐、2-甲基雷锁辛和N,N-二乙基甲苯-2,5-二胺HCl外,对其他各组分的含量测定值的相对标准偏差(RSD)均小于10%,加标回收率为77.6%～122.8%。该方法简便、准确、快速,适于氧化型染发剂中染料成分的检测。

Determination of 22 components in hair dyes by high performance liquid chromatography

A high performance liquid chromatographic (HPLC) method was developed for the determination of 22 components in hair dyes. The target analytes were separated on an amide bonded C16 silica column (250 mm×4.6 mm, 5 μm), employing acetonitrile-0.025 mol/L phosphate buffer (pH 6.0) containing 0.1% 1-heptanesulfonic acid sodium salt as the mobile phase and detected by a photodiode array detector (DAD) with the detection wavelengths of 260 nm and 280 nm. The flow rate was 1.0 mL/min and the column temperature was 25 ℃. The linear range was from 10 mg/L to 500 mg/L with good relationship. The relative standard deviations were less than 10%(except toluene-2,5-diamine sulfate, 2-methylresorcinol, N,N-diethyltoluene-2,5-diamine HCl at low concentrations) and the recoveries were from 77.6% to 122.8%. The method is simple, rapid and accurate, and is suitable for the analysis of various hair dyes.