DSC examination of intestinal tissue following warm ischemia and reperfusion injury |
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Authors: | Klára Nedvig Andrea Ferencz Erzsébet Rőth D. Lőrinczy |
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Affiliation: | 1.Department of Surgical Research and Techniques, Medical School,University of Pécs,Pécs,Hungary;2.Institute of Biophysics, Medical School,University of Pécs,Pécs,Hungary |
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Abstract: | The fact that small bowel is extremely sensitive to ischemia/reperfusion (I/R) injury had encouraged us to compare the conventional histology and differential scanning calorimetry (DSC) methods in intestinal structural changes following experimental warm I/R models. Our histological findings showed that longer warm I/R period caused more severe damage in structure of mucosa and crypts, but there were no changes in the muscular layer. According to our DSC data (transition temperature, calorimetric enthalpy) suggest that the thermal destruction of mucosa, muscular layer and total intestinal wall following I/R injury revealed significant differences compared to normal bowel structure. |
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