鸡蛋中三聚氰胺的液相色谱快速测定

建立了鸡蛋中三聚氰胺的液相色谱快速检测方法。样品经1%三氯乙酸溶液超声提取,磺基水杨酸和乙腈沉淀蛋白,高速离心分离,上清液过滤后直接由高效液相色谱分析。在优化的条件下,样品的色谱分析时间仅为10 min;三聚氰胺标准品在0.05～20 mg/L范围内,线性相关系数为0.9999;方法定量限为0.10 mg/kg (S/N＞10);1.0,2.0,4.0,6.0 mg/kg 4个加标水平测试,回收率在94.2%～107%之间;6次平行测试的相对标准偏差(RSD)为1.53%～2.06%。该方法前处理简单、分析速度快、灵敏度高,满足鸡蛋中三聚氰胺检验工作的快速测定要求。

Rapid determination of melamine in eggs by high performance liquid chromatography

A rapid method for the determination of melamine in eggs was established using high performance liquid chromatography (HPLC). The melamine in a test sample was extracted with ultrasonic in 1% trichloroacetic acid for 10 min, and then purified with precipitation, centrifugation and filtration, at last tested by HPLC system. The total time of HPLC analysis for each sample was only 10 min. The linear range was from 0.05 to 20.0 mg/L as the linear correlation coefficient was 0.9999. The limit of quantification was 0.10 mg/kg (S/N＞10). The average recoveries were between 94.2% and 107% in the spiked range of 1.0~6.0 mg/kg in eggs. The relative standard deviations (RSDs) of parallel determination were between 1.53% and 2.06%(n6). This method is simple, fast, sensitive and suitable for the determination of melamine in eggs.