Preparative HPLC for analysis of mineralocorticoids in human plasma |
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Authors: | C Lejeune-Lenain S Kina D Bosson |
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Institution: | 1. Chimie Médicale, H?pital Saint-Pierre, Université Libre de Bruxelles, rue Haute, 322, 1000, Bruxelles, Belgium
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Abstract: | Summary A method has been developed to measure simultaneously in human plasma the mineralocorticoids and glucocorticoids involved
in mineralocorticoid excess.
Briefly: plasma extracts were chromatographied in a fully-automated procedure on a lichrospher-diol column using a hexane:
isoproanol: trithylamine gradient. Eluates were collected on a preprogrammed fraction collector. Final quantification was
achieved by RIA on the selected fractions.
This procedure allowed: 1) the separation of each measured steroid from plasma contaminants; 2) precise collection; 3) quantification
of mineralocorticoids with a precision and sensitivity equivalent to that of direct methods; and 4) improved specificity. |
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Keywords: | HPLC-RIA Mineralocorticoids Glucocorticoids Adrenal steroids |
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