Time-resolved fluoroimmunoassay for ractopamine in swine tissue |
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Authors: | Jianzhong Shen Zhen Zhang Yan Yao Weimin Shi Yibing Liu Suxia Zhang |
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Institution: | (1) Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China;(2) Department of Isotope, China Institute of Atomic Energy, Beijing, 102413, China |
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Abstract: | This study describes the development and validation of a time-resolved fluoroimmunoassay (TR-FIA) for screening ractopamine
(RAC) in swine tissue. The method is based on the direct competitive-type immunoassay using europium-labeled anti-RAC monoclonal
antibody as a tracer and RAC–ovalbumin as a solid-phase antigen. When RAC was spiked at levels of 1–10 μg kg−1, recoveries ranged from 88.2 to 118.5% for swine liver and muscle with coefficients of variation from 7.1 to 20.5%. The detection
limit was 0.1 μg kg−1. The proposed TR-FIA method was applied to the determination of RAC in an actual residue study and the applicability was
confirmed by liquid chromatography–tandem mass spectrometry. |
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Keywords: | Time-resolved fluoroimmunoassay Ractopamine Swine tissue |
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