阿胶、龟甲胶中脂溶性成分的高效液相色谱指纹图谱

采用高效液相色谱法研究并建立东阿阿胶、龟甲胶脂溶性成分的指纹图谱,为药用动物胶的质量控制提供了有效的方法。采用液-液-液三相静态萃取方法制备样品,以水-乙腈为流动相进行二元梯度洗脱,检测波长为205 nm,柱温25 ℃,分析时间为60 min。采集20批样品的色谱图并对其进行相似度和聚类分析。分别标定了阿胶、龟甲胶的共有峰,其相似度分析及聚类分析结果显示两种胶间存在着明显的差异。该方法稳定可靠,可以有效地区别不同种属的药用动物胶,为动物胶剂的鉴别及质量控制提供了依据。

Development of high performance liquid chromatographic fingerprints of liposoluble constituents in donkey- hide glue and tortoise-shell glue

A sensitive and specific method with high performance liquid chromatographic fingerprints was developed for controlling the quality of donkey-hide glue and tortoise-shell glue according to the liposoluble constituents. The samples were prepared with the liquid-liquid-liquid three-phase static extraction. The liposoluble constituents were analysed by reversed-phase high performance liquid chromatography with a gradient program and detected at 205 nm. The chromatograms were evaluated by similarity and cluster analysis. The common peaks of two glues were marked respectively. The differences between two glues were revealed by similarity and cluster analysis. The method has good repeatability and stability, and can be served as the means to identify different kinds of animal glue.