| HUMAN CHROMOSOME BANDING WITH RESTRICTION ENDONUCLEASES HaeIII,HinfI AND PstI |
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| 作者姓名： | 张思仲 董伟峰 |
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| 作者单位： | Department of Medical Genetics，West China University of Medical Sciences，Chengdu 610044，PRC，Department of Medical Genetics，West China University of Medical Sciences，Chengdu 610044，PRC |
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| 基金项目： | Project supported by National Natural Science Foundation of China. |
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| 摘 要： | Human chromosome banding was carried out with restriction endonucleases (REs)HaeⅢ, HinfⅠ, PstⅠ and the effects of some factors on it were examined. HaeⅢ induced Cand G banding patterns, HinfⅠ induced negative C bands but the centromeric heterochro-matin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphismof C banded region was discovered in chromosome 4. PstⅠ induced G-like banding pattern.The duration of enzymatic digestion, the concentration of glycerol in the reaction buffer,as well as the ageing and heating of chromosome preparations all had a significant influ-ence on the banding effects of the REs.
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HUMAN CHROMOSOME BANDING WITH RESTRICTION ENDONUCLEASES HaeIII,HinfI AND PstI |
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| ZHANG SI-ZHONG,DONG WEI-FENG.HUMAN CHROMOSOME BANDING WITH RESTRICTION ENDONUCLEASES HaeIII,HinfI AND PstI[J].Science in China(Chemistry),1989(11). |
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| Authors: | ZHANG SI-ZHONG DONG WEI-FENG |
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| Institution: | ZHANG SI-ZHONG,DONG WEI-FENG Department of Medical Genetics,West China University of Medical Sciences,Chengdu 610044,PRC |
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| Abstract: | |
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| Keywords: | restriction endonucleases human chromosome banding |
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