2',3'-Dideoxy-3'-thionucleoside triphosphates: syntheses and polymerase substrate activities |
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Authors: | Meena Sam Mui Pierce Kathryn Szostak Jack W McLaughlin Larry W |
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Institution: | Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA 02467, USA. |
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Abstract: | All four 2',3'-dideoxy-3'-thio-nucleosides (ddtNTPs) function as substrates for the Y410F mutant of Deep Vent (exo-) DNA polymerase. Not only are the ddtNTPs incorporated to form the N + 1 product, but further elongations are observed in which the key step is attack of the 3'-thiol on the 5'-triphosphate. Although other polymerases are likely to differ in their use of the ddtNTPs, there does not appear to be a fundamental prohibition against using a thiol nucleophile on a phosphate anhydride electrophile. The syntheses of four ddtNTPs (C, T, A, G) are described. structure: see text] |
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