| 胶体金免疫层析法快速检测腹泻性贝毒软海绵酸的研究 |
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| 引用本文： | 刘仁沿,梁玉波,陈媛,许道艳,陈冰君,刘永健. 胶体金免疫层析法快速检测腹泻性贝毒软海绵酸的研究[J]. 分析科学学报, 2010, 26(1). DOI: 10.3969/j.issn.1006-6144.2010.01.007 |
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| 作者姓名： | 刘仁沿 梁玉波 陈媛 许道艳 陈冰君 刘永健 |
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| 作者单位： | 国家海洋环境监测中心,辽宁大连,116023;江西中德生物工程有限公司,江西南昌,330029 |
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| 基金项目： | 国家863项目(No.2006AA09Z163);;国家专项(No.908-01-ZH3);;国家自然科学基金(No.40876055) |
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| 摘 要： | 腹泻性贝毒是一类分布较广的赤潮毒素,严重威胁到人类的健康和安全。本文用胶体金标记利用细胞融合技术制备的抗软海绵酸单克隆抗体,使用卵清蛋白合成高偶联比的包被抗原,以硝酸纤维素膜为载体,利用免疫层析技术原理,建立了快速检测软海绵酸的免疫层析试纸条方法。方法检出限12 ng/mL(0.96纳克/条)。
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| 关 键 词： | 软海绵酸 单克隆抗体 免疫层析 胶体金 |
Development of Gold Immunochromatography Assay for he Detection of Okadaic Acid |
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| LIU Ren-yan,LIANG Yu-bo,CHEN Yuan,XU Dao-yan,HEN Bing-jun,LIU Yong-jian. Development of Gold Immunochromatography Assay for he Detection of Okadaic Acid[J]. Journal of Analytical Science, 2010, 26(1). DOI: 10.3969/j.issn.1006-6144.2010.01.007 |
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| Authors: | LIU Ren-yan LIANG Yu-bo CHEN Yuan XU Dao-yan HEN Bing-jun LIU Yong-jian |
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| Affiliation: | 1.National Marine Environmental Monitoring Center;Dalian 116023;2.Zodolabs Biotech CO.;LTD.;Nanchang 330029 |
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| Abstract: | A competitive gold immunochromatography assay for okadaic acid(OA) was developed in this paper.The colloidal gold particles were coated with purified monoclonal anti-OA antibodies prepared by cell fusion.The capture reagent in the assay was an OA-ovalbumin conjugate immobilized on the lateral flow membrane of the test device.The detection limit of the rapid test strip was 12 ng/mL.The method was fast and simple.The results could be obtained within 15 min and all reagents were included in the test device.Oth... |
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| Keywords: | Akadaic acid Monoclonal antidoby Immunochromatography Colloidal particles |
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